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Blue Cross Bromsgrove rehoming centre, Bromsgrove. 11,320 likes · 141 talking about this · 986 were here. The Bromsgrove centre opened in 1986 and is now one of our largest centres. Located in...In brief, size selection was performed on genomic DNA from the two affected individuals using Blue Pippin (cassette kit: BUF7510; size range: 30–40 kb). After assessment of DNA integrity and concentration, the library was prepared with a SQK-LSK108 library preparation kit and sequenced using PromethION sequencing. The data from PromethION ...The Apple Pippin is a defunct open multimedia technology platform, designed by Apple Computer, and marketed as PiPP!N. According to Apple, Pippin was directed at the home market as "an integral part of the consumer audiovisual, stereo, and television environment." ... Sonoran Blue 1996 NA, JP WebViewer with MS Internet Explorer: Non-game, Web ...They are all embellished with the same fanciful Early Bird pattern, consisting of a light blue background, adorned with a bird and exotic flowers. The porcelain ...

To anchor the scaffolds to chromosomes, 26.79 Gb of clean reads (65.50×) were obtained via Hi-C library sequencing (Supplemental Table 5).Read pairs were mapped to the draft assembly using BWA (version .7.10-r789) (Li and Durbin, 2009).We determined that 85.28% of the reads were correctly mapped to the genome (Supplemental Table 6), including 22.01% uniquely mapped read pairs.For PacBio libraries, we followed the study of Chakraborty et al. by shearing the DNA using a 1.5-inch 24-gauge blunt-tipped needle, and the library was prepared using PacBio's SMRTbell template protocol and Blue Pippin size selection using a 15- to 50-kb cutoff. We generated several dozen libraries in this manner and collected 124 total ...Blue Pippin. The platform features the ability for targeted size selection of DNA fragments (90bp-50kb) with minimal effort for the purpose of Next-Generation Sequencing. Target sizes or ranges of sizes are entered in software, and fractions are collected in buffer. Up to 5 samples per gel cassette may be run, with no possibility of cross ...DNA size-selection was performed using Blue Pippin 2% dye free cassette with internal standard marker V1 (Sage Science). The size-selected product was equalized using the Ion Equalizer kit (LifeTechnologies, ThermoFisher Scientific) following manufacturer's instructions. All purifications used Agencourt AMPure XP Reagent (Beckman Coulter).Selection Chart Pippin Prep BluePippin PippinHT Max. DNA input 5 μg 5 μg 1.5 μg Max. Sample Capacity 5 5 24 Pulsed-Field No Yes Yes Run Times (100 bp - 1.5 kb) 50-90 min 50-90 min* 25-45 min* Size Selection Range 100 bp - 1.5 kb 100 bp - 50 kb 100 bp - 1.5 kb High-Pass Size Filtering No Yes Yes Low-Pass Size Filtering No No Yes Specifications*

Nike Air Pippen 1 Work Blue Basketball Shoes, #325001-403, Leather, Men's US 15. $125.88. $14.95 shipping. or Best Offer. 10 watching. Authenticity Guarantee. Kith x Nike Air Pippen 1 Chimera rare Sz 6 Animal Print uptempo max AH1070 900. $175.00. $14.95 shipping. or Best Offer.BluePippin Operations Manual 460013 Rev B 1-2 1.2 How the System Works The BluePippin systems use pre-cast and disposable agarose gel cassettes.BluePippin and Pippin Prep Rinse Cassette. Sage Science SKU: SAG-720040. Price: $55. ….

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Automated, preparative gel electrophoresis systems, used for accurate and reproducible size selection/collection of DNA (or cDNA) fragments, for the preparation of libraries for next generation sequencing (NGS) The supplier does not provide quotations for this product through SelectScience. Browse these related products instead.The Sage Science Pippin Pulse is an affordable tool allowing researchers to resolve DNA up to 430 kb. Pulsed-Field Technology. Utilises innovative pulsed field inversion electrophoresis that works by shuttling DNA back and forth in the gel, effectively slowing down large DNA fragments that would typically migrate at the same rate as smaller DNA ...

Blue Pippin nucleic acid size selection; Please see the Getting Started page to learn how to work with our Core. Please also see our Instrument List. We encourage you to consult our extensive FAQs in case of questions. Illumina has posted NGS tutorials in this “Beginner’s Guide to NGS”. Our Shared Equipment includes:Libraries are run through 10-15 cycles of PCR with Kapa Biosystems Library Amplification kits, followed by further size selection with Blue Pippin Prep (Sage Science). Final library quantification is achieved using the Qubit Fluorometer (for concentration) and the Agilent 2100 Bioanalyzer (for library insert size and length).PULSE1: Pippin Pulse Starter Bundle1, with Gel Box, Notebook, and KBB Buffer

states gdp per capita ranking For libraries targeted at 12 kb, we carried out size selection for sheared fragments larger than 10 kb using a 0.75% agarose cassette (Sage Science; BLF7510) with the Blue Pippin DNA size ... missouri state vsbetty boop catch phrase Sage Science bluepippin device Bluepippin Device, supplied by Sage Science, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and moreThe Pippin platform is typically used to construct genomic libraries for next-gen sequencing. The BluePippin is an updated version of Sage's Pippin Prep system, to which a pulsed-field power ... planet fitness hours 4th of july Double digest restriction-site associated sequencing (ddRAD-seq) is a flexible and cost-effective strategy for providing in-depth insights into the genetic architecture of germplasm collections. sigma nu kureeves austin2 00 pm eastern Aug 27, 2013 · In order to measure the increase in subread length, long insert libraries were prepared with fragments larger than 4 kb or 7 kb isolated using the Blue Pippin and a 0.75% Agarose Gel Cassette (BLF7510) and compared to a library without Blue Pippin size selection. quincy acy raptors For Nanopore sequencing, large fragments of genomic DNA were obtained using the Blue Pippin automatic nucleic acid fragment recovery system (Sage Science, MA), and barcodes were added using the Nanopore EXP-NBD104 kit. kansas stats basketballkansas counties mappre major Sequencing of samples was performed using MiSeq Reagent Kit v3 (600 cycle; 2-300 bp) (Illumina, San Diego, CA, USA). For Long-read WGS, the library preparation and size selection were performed using PacBio Template Preparation 1.0 kit (Pacific Biosciences, Menlo Park, CA, USA) and Blue Pippin (Sage Sciences, Beverly, MA, USA), respectively.